Additional Selective and Differential Cell Culture Media
NEOGEN Dextrose Tryptone Broth, 5 kg
Dextrose Tryptone Broth is used for cultivation of mesophilic or thermophilic spoilage microorganisms from food in a laboratory setting. Dextrose Tryptone Broth is not intended for use in the diagnosis of disease or other conditions in humans.In the 1930’s, the National Canners Association specified use of Dextrose Tryptone Agar for isolating “flat sour” organisms from food products. “Flat sour” spoilage of canned foods is caused by Bacillus coagulans (Bacillus thermoacidurans). Bacterial growth results in a 0.3 – 0.5 drop in pH, while ends of the can remain flat. B. coagulans is a soil microorganism and found in canned tomato products and dairy products. Conditions favorable for multiplication of the bacterium can result in spoilage of the food product.For more information visit https://www.neogen.com/solutions/Microbiology/dextrose-tryptone-broth
NEOGEN KF Streptococcus Agar, 500 g
KF Streptococcus Agar is used with triphenyltetrazolium chloride for the selective isolation and enumeration of fecal streptococci in a laboratory setting. KF Streptococcus Agar is not intended for use in the diagnosis of disease or other conditions in humans.KF Streptococcus Agar was developed by Kenner, Clark, and Kabler for the detection of fecal streptococci in surface waters. KF Streptococcus Agar is currently used in detecting fecal streptococci in water, foods, and other materials through the membrane filtration technique or pour plate method. Fecal streptococci are normal inhabitants in the intestines, and the presence of fecal streptococci can be used as an indication of fecal contamination. The presence of fecal streptococci is of value in determining pollution sources because certain fecal streptococci are host-specific.For more information visit https://www.neogen.com/solutions/Microbiology/kf-streptococcus-agar
NEOGEN Brain Heart Infusion Agar, 500 g
Brain Heart Infusion Agar is used for the cultivation of a wide variety of fastidious organisms and is not intended for use in the diagnosis of disease or other conditions in humans.A general purpose nutritious agar base. This medium was first used for the isolation of dental pathogens. With the addition of 7% defibrinated blood the medium will support the growth of a wide range of fastidious organisms, the phosphate buffer will help neutralize the acids produced from the utilization of glucose and thus maintain viability. The medium is not recommended for the determination of hemolytic reactions because of the glucose content.
For more information visit https://www.neogen.com/solutions/Microbiology/brain-heart-infusion-agar
NEOGEN Perfringens Agar Base (TSC), 10 kg
Perfringens Agar Base (TSC) is used with selective and differential supplements for the recovery of Clostridium perfringens, and is not intended for use in the diagnosis of disease or other conditions in humans. Formulated according to ISO 14189:2013, Perfringens Agar Base (TSC) is a nutritious base medium used in the preparation of SFP Agar and TSC Agar. Depending upon the formula, supplements are added to increase the selectivity of the medium. Shahidi-Ferguson Perfringens (SFP) Agar is based on the formula by Shahidi and Ferguson and incorporates the supplements, Polymyxin B Sulfate and Kanamycin. Tryptose Sulphite Cycloserine (TSC) Agar is developed with the same base as SFP Agar, with the addition of Cycloserine. If desired, Egg Yolk Emulsion can be added to either formula.For more information visit https://www.neogen.com/solutions/Microbiology/perfringens-agar-base
NEOGEN Tetrathionate Broth Base, 500 g
Tetrathionate Broth Base is used with iodine for the recovery of Salmonella spp. in a laboratory setting. Tetrathionate Broth Base is not intended for use in the diagnosis of disease or other conditions in humans. Tetrathionate Broth Base is used as a selective enrichment for the cultivation of Salmonella spp. Salmonella organisms may be injured in food-processing procedures, which include exposure to low temperatures, sub-marginal heat, drying, radiation, preservative, and sanitizers. Mueller demonstrated the effectiveness of Tetrathionate Broth for enriching typhoid and paratyphoid bacilli while inhibiting coliform organisms. Using modified Mueller’s broth, Kauffmann increased the number of positive isolates. Tetrathionate Broth was used in studies for the poultry industry and in a collaborative study for rapid screening of Salmonella in food.For more information visit https://www.neogen.com/solutions/Microbiology/tetrathionate-broth-base
NEOGEN Phosphate Buffer, pH 7.2, 5 kg
Phosphate Buffer, pH 7.2 is used for the preparation of microbiological dilution blanks in a laboratory setting. Phosphate Buffer, pH 7.2 is not intended for use in the diagnosis of disease or other conditions in humans. The formula for phosphate buffer is specified by the American Public Health Association (APHA) for use in diluting test samples. Phosphate Buffer, pH 7.2 is specified for use in diluting water, dairy products and food for microbiological methods. In the examination of water and dairy products the addition of magnesium chloride is recommended. This buffer is also referred to as Butterfield’s Buffered Phosphate Diluent and recommended for examination of food. Phosphate Buffer, pH 7.2 stabilizes the pH of water used for dilutions.For more information visit https://www.neogen.com/solutions/Microbiology/phosphate-buffer-ph-72
NEOGEN Agar No. 4 - Plant Tissue Culture Grade, 500 g
Agar No.4 has been selected specifically for use as a gelling agent in plant tissue culture techniques, and is not intended for use in the diagnosis of disease or other conditions in humans.Agar No.4 is selected primarily on gel strength, a parameter of particular importance for plant tissue culture techniques, and then tested to ensure it meets the parameters set by a major Horticultural company. The agar contains no nutrients for plant growth and is designed to be incorporated into classical formulations such as Murashige and Skoog, as well as customer’s own formulations.For more information visit https://www.neogen.com/solutions/Microbiology/agar-number-4-plant-tissue-culture-grade
NEOGEN Kanamycin Aesculin Azide Agar, 500 g
Kanamycin Aesculin Azide Agar is a selective isolation and enumeration medium for enterococci (Lancefield group D streptococci) in food, and is not intended for use in the diagnosis of disease or other conditions in humans.Sodium azide and kanamycin provide the selective inhibition required whilst aesculin and iron salts form an indicator system for the presumptive identification of enterococci. Incubation at 42°C will increase the medium’s selectivity.Formula:Tryptone - 20.0 g/LYeast Extract - 5.0 g/LSodium Chloride - 5.0 g/LSodium Citrate - 1.0 g/LAesculin - 1.0 g/LFerric Ammonium Citrate - 0.5 g/LSodium Azide - 0.15 g/LKanamycin Sulphate - 0.02 g/LAgar- 10.0 g/LFor more information visit https://www.neogen.com/solutions/Microbiology/kanamycin-aesculin-azide-agar
NEOGEN Tryptone No. 1, 10 kg
Tryptone No. 1 is an enzymatic digest of casein and meat for use in preparing microbiological culture media in a laboratory setting. Tryptone No. 1 is not intended for use in the diagnosis of disease or other conditions in humans. Tryptone No. 1 is an enzymatic digest of casein and meat used as a nitrogen source in culture media. Casein is the main protein of milk, and a rich source of amino-acid nitrogen. Tryptone No. 1 is rich in tryptophane, making it valuable for use in detecting indole production. The absence of detectable levels of carbohydrates in Tryptone makes it a suitable peptone in differentiating bacteria on the basis of their ability to ferment various carbohydrates. Several media containing Tryptone are specified in standard methods for multiple applications.For more information visit https://www.neogen.com/solutions/Microbiology/tryptone-number-1
NEOGEN Terrific Broth, Modified, 10 kg
Terrific Broth, Modified is used with glycerol in cultivating recombinant strains of Escherichia coli in a laboratory setting. Terrific Broth, Modified is not intended for use in the diagnosis of disease or other conditions in humans. Terrific Broth, Modified was developed by Tartoff and Hobbs to increase yields of plasmid DNA from transformed E. coli and is a popular choice of media available for plasmid preps. While cells grown in Terrific Broth, Modified can grow slower than other liquid media, the final cell density and plasmid yields are 2 – 4 fold higher. The higher yield is a result of the increased concentration of Enzymatic Digest of Casein and Yeast Extract in this formula. Glycerol is used as a carbohydrate source, and unlike glucose, is not fermented to acetic acid.For more information visit https://www.neogen.com/solutions/Microbiology/terrific-broth-modified
NEOGEN Skim Milk Powder, 10 kg
Skim Milk Powder is used when preparing microbiology culture media in a laboratory setting. Milk Powder is not intended for use in the diagnosis of disease or other conditions in humans. A bacteriological grade of thermophile free spray dried skim milk. Used in Milk Plate Count Agar NCM0119 and in media for diagnostic tests involving the digestion or coagulation of casein and the fermentation of lactose. Recommended working concentration 10%.For more information visit https://www.neogen.com/solutions/Microbiology/skim-milk-powder
NEOGEN Slanetz and Bartley Agar, 500 g
Slanetz and Bartley Medium is used for the enumeration of enterococci from water samples as described in ISO 7899-2, and is not intended for use in the diagnosis of disease or other conditions in humans. This medium was originally described by Slanetz and Bartley for the enumeration of enterococci from water samples using a membrane filtration technique, but it may also be used as a spread plate for the examination of other sample types. Enterococci reduce tetrazolium chloride to the insoluble red dye formazan, producing colonies which are dark red or maroon on the surface of the membrane or agar. This reaction is not exclusive to enterococci, and the count at this stage should be considered presumptive. Colonies may be confirmed as enterococci by demonstrating aesculin hydrolysis using Kanamycin Aesculin Azide Agar (NCM0198).For more information visit https://www.neogen.com/solutions/Microbiology/slanetz-bartley-medium
NEOGEN EC Medium, Modified with Novobiocin, 5 kg
EC Medium, Modified with Novobiocin is used for the selective enrichment of Escherichia coli O157:H7 in a laboratory setting. EC Medium, Modified with Novobiocin is not intended for use in the diagnosis of disease or other conditions in humans.EC Medium was developed by Hajna and Perry in an effort to improve the methods for the detection of the coliform group and E. coli. This medium consists of a buffered lactose broth with the addition of 0.15% Bile Salts Mixture. Growth of spore-forming bacteria and fecal streptococci were inhibited by the bile salts.EC Medium, Modified with the addition of Novobiocin was first described by Okrend and Rose. Okrend and Rose modified EC Medium by reducing the Bile Salts Mixture concentration to 1.12% and adding 20 mg/L of sodium novobiocin.For more information visit https://www.neogen.com/solutions/Microbiology/ec-medium-modified-with-novobiocin
NEOGEN Bacteriological Peptone, 5 kg
Bacteriological Peptone is for use in preparing microbiological culture media in a laboratory setting, and is not intended for use in the diagnosis of disease or other conditions in humans.An economical source of nutrients provided by a balanced mixture of meat peptones and tryptone. The growth requirements of most non-fastidious organisms will be fulfilled by the range of amino acids, peptides and proteoses in this mixture.For more information visit https://www.neogen.com/solutions/Microbiology/bacteriological-peptone
NEOGEN LB Broth, Lennox, 500 g
LB Broth, Lennox is used in molecular genetic studies in a laboratory setting. LB Broth, Lennox is not intended for use in the diagnosis of disease or other conditions in humans.LB Broth formula is based on L Broth described by Lennox for growth and maintenance of E. coli strains used in molecular microbiology procedures. LB Broth is nutritionally rich, formulated for the isolation of pure recombinant strains. E. coli is grown to late log phase in LB Broth. Some plasmid vectors may replicate to high copy numbers without selective amplification. Some vectors may require selective amplification to reach high copy numbers. Chloramphenicol can be added to inhibit host synthesis and, as a result, prevent replication of the bacterial chromosome.For more information visit https://www.neogen.com/solutions/Microbiology/lb-broth-lennox